Category: publications

Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on αv Integrins

Gallo E, Kelil A, Haughey M, Cazares-Olivera M, Yates BP, Zhang M, Wang NY, Blazer L, Carderelli L, Adams JJ, Kossiakoff AA, Wells JA, Xie W, Sidhu SS

J Mol Biol. 2021 Jun 4;433(15):167090. doi: 10.1016/j.jmb.2021.167090.

PMID: 34090922

Members of the αv family of integrins regulate activation of …

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Structural Insights into PLCε: Discovery of an Integrated RA1 Domain and Novel Regulatory Elements

Garland E, Rugema N, Sieng M, Muralidharan K, Fisher I, Everly A, Erramilli S, Van Camp M, Emmerson L, Reyes J, Kossiakoff AA, Lyon, A

The FASEB Journal, 35. 2021 May 14. https://doi.org/10.1096/fasebj.2021.35.S1.03032

Phospholipase C (PLC) ε enzymes hydrolyze phosphatidylinositol lipids at cellular membranes to produce inositol phosphates (IPx) and …

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Engineered ultra-high affinity synthetic antibodies for SARS-CoV-2 neutralization and detection

Slezak T, Kossiakoff AA

J Mol Biol. 2021 Mar 25;doi: 10.1016/j.jmb.2021.166956.

PMID: 33775667

Abstract

The Covid-19 pandemic is a centenarial global catastrophe. Similar events are likely to be recurring with more frequency in the future. The inability to control the virus’ impact is caused by many …

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Recognition of an α-helical hairpin in P22 large terminase by a synthetic antibody fragment

Lokareddy RK, Ko YH, Hong N, Doll SG, Paduch M, Niederweis M, Kossiakoff AA, Cingolani G

Acta Crystallogr D Struct Biol. 2020 Sep 1;76(Pt 9):876-888.

PMID: 32876063

Abstract

The genome-packaging motor of tailed bacteriophages and herpesviruses is a multisubunit protein complex formed by several copies of …

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Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling

Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC.

Elife. 2020 Aug 7;9:e58464.

PMID: 32762848

Abstract

Frizzleds (Fzd) are the primary receptors for Wnt morphogens, which are essential regulators of stem cell biology, yet …

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Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins

Mukherjee S, Erramilli SK, Ammirati M, Alvarez FJD, Fennell KF, Purdy MD, Skrobek BM, Radziwon K, Coukos J, Kang Y, Dutka P, Gao X, Qiu X, Yeager M, Eric Xu H, Han S, Kossiakoff AA

Nat Commun 2020 Mar;11(1):1598

PMID: 32221310

Abstract

We propose the concept of universal fiducials based on a set of pre-made …

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Structure and mechanism of the ER-based glucosyltransferase ALG6

Bloch JS, Pesciullesi G, Boilevin J, Nosol K, Irobalieva RN, Darbre T, Aebi M, Kossiakoff AA, Reymond JL, Locher KP

Nature 2020 03;579(7799):443-447

PMID: 32103179

Abstract

In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins. The final seven steps occur in the lumen …

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Structure and drug resistance of the Plasmodium falciparum transporter PfCRT

Kim J, Tan YZ, Wicht KJ, Erramilli SK, Dhingra SK, Okombo J, Vendome J, Hagenah LM, Giacometti SI, Warren AL, Nosol K, Roepe PD, Potter CS, Carragher B, Kossiakoff AA, Quick M, Fidock DA, Mancia F

Nature 2019 Nov;

PMID: 31776516

Abstract

The emergence and spread of drug-resistant Plasmodium falciparum impedes global efforts to control …

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Development of “Plug and Play” Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM

Dutka P, Mukherjee S, Gao X, Kang Y, de Waal PW, Wang L, Zhuang Y, Melcher K, Zhang C, Xu HE, Kossiakoff AA

Structure 2019 Oct;

PMID: 31669042

Abstract

“Universal” synthetic antibody (sAB)-based fiducial marks have been generated by customized phage display selections to facilitate the rapid structure determination of G protein-coupled receptor (GPCR) signaling …

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An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability

Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA

Protein Sci. 2019 Oct;

PMID: 31622515

Abstract

Engineered recombinant antibody-based reagents are rapidly supplanting traditionally derived antibodies in many cell biological applications. A particularly powerful aspect of these engineered reagents is that other modules having myriad functions can be attached to …

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