Category: publications

Structure of an AMPK complex in an inactive, ATP-bound state

Yan Yan, Somnath Mukherjee, Kaleeckal G. Harikumar, Timothy S. Strutzenberg, Edward Zhou, Kelly Suino-Powell, Ting-Hai Xu, Ryan D. Sheldon, Jared Lamp, Joseph S. Brunzelle, Katarzyna Radziwon, Abigail Ellis, Scott J. Novick, Irving E. Vega, Russell G. Jones, Laurence J. Miller, H. Eric Xu, Patrick R. Griffin, Anthony A. Kossiakoff, Karsten Melcher

Science …

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Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1

Qiuyan Chen, Manolo Plasencia, Zhuang Li, Somnath Mukherjee, Dhabaleswar Patra, Chun-Liang Chen, Thomas Klose, Xin-Qiu Yao, Anthony A Kossiakoff, Leifu Chang, Philip C Andrews, John J G Tesmer

Nature. 2021 Jul 14. doi: 10.1038/s41586-021-03721-x

PMID: 34262173

G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated GPCRs, thereby priming them …

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K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle

Yuanyuan Yu, Qingyun Zheng, Satchal K. Erramilli, Man Pan, Seongjin Park, Yuan Xie, Jingxian Li, Jingyi Fei, Anthony A. Kossiakoff, Lei Liu & Minglei Zhao

Nat Chem Biol. 2021 Jul 8. doi: 10.1038/s41589-021-00823-5

PMID: 34239127

Protein ubiquitination shows remarkable topological and functional diversity through the polymerization of ubiquitin …

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A T cell redirection platform for co-targeting dual antigens on solid tumors

Leonie Enderle, Karim H Shalaby, Maryna Gorelik, Alexander Weiss, Levi L Blazer, Marcin Paduch, Lia Cardarelli, Anthony Kossiakoff, Jarrett J Adams, Sachdev S Sidhu

MAbs. Jan-Dec 2021;13(1):1933690. doi: 10.1080/19420862.2021.1933690

PMID: 34190031

In order to direct T cells to specific features of solid cancer cells, we engineered a bispecific …

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Structural basis of omega-3 fatty acid transport across the blood-brain barrier

Rosemary J Cater, Geok Lin Chua, Satchal K Erramilli, James E Keener, Brendon C Choy, Piotr Tokarz, Cheen Fei Chin, Debra Q Y Quek, Brian Kloss, Joseph G Pepe, Giacomo Parisi, Bernice H Wong, Oliver B Clarke, Michael T Marty, Anthony A Kossiakoff, George Khelashvili, David L Silver, Filippo Mancia

Nature volume …

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Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody that Targets two Distinct Epitopes on αv Integrins

Gallo E, Kelil A, Haughey M, Cazares-Olivera M, Yates BP, Zhang M, Wang NY, Blazer L, Carderelli L, Adams JJ, Kossiakoff AA, Wells JA, Xie W, Sidhu SS

J Mol Biol. 2021 Jun 4;433(15):167090. doi: 10.1016/j.jmb.2021.167090.

PMID: 34090922

Members of the αv family of integrins regulate activation of …

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Engineered ultra-high affinity synthetic antibodies for SARS-CoV-2 neutralization and detection

Slezak T, Kossiakoff AA

J Mol Biol. 2021 Mar 25;doi: 10.1016/j.jmb.2021.166956.

PMID: 33775667

Abstract

The Covid-19 pandemic is a centenarial global catastrophe. Similar events are likely to be recurring with more frequency in the future. The inability to control the virus’ impact is caused by many …

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Recognition of an α-helical hairpin in P22 large terminase by a synthetic antibody fragment

Lokareddy RK, Ko YH, Hong N, Doll SG, Paduch M, Niederweis M, Kossiakoff AA, Cingolani G

Acta Crystallogr D Struct Biol. 2020 Sep 1;76(Pt 9):876-888.

PMID: 32876063

Abstract

The genome-packaging motor of tailed bacteriophages and herpesviruses is a multisubunit protein complex formed by several copies of …

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Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling

Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC.

Elife. 2020 Aug 7;9:e58464.

PMID: 32762848

Abstract

Frizzleds (Fzd) are the primary receptors for Wnt morphogens, which are essential regulators of stem cell biology, yet …

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Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins

Mukherjee S, Erramilli SK, Ammirati M, Alvarez FJD, Fennell KF, Purdy MD, Skrobek BM, Radziwon K, Coukos J, Kang Y, Dutka P, Gao X, Qiu X, Yeager M, Eric Xu H, Han S, Kossiakoff AA

Nat Commun 2020 Mar;11(1):1598

PMID: 32221310

Abstract

We propose the concept of universal fiducials based on a set of pre-made …

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