Engineered Protein-G variants for plug-and-play applications

Tomasz Slezak, Kelly M. O’Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. Kossiakoff

bioRxiv 2024 August 08.

PMID: 39211271 PMCID: PMC11361062 DOI: 10.1101/2024.08.06.606809

We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful …

Continue reading

Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Jane E. Lodwick, Rong Shen, Satchal Erramilli, Yuan Xie, Karolina Roganowicz, Anthony A. Kossiakoff, Minglei Zhao

bioRxiv 2024 Jun 27:2024.06.27.601040. doi: 10.1101/2024.06.27.601040.

PMID: 38979142 PMCID: PMC11230398 DOI: 10.1101/2024.06.27.601040

Vault is a massive ribonucleoprotein complex found across Eukaryota. The major vault protein (MVP) oligomerizes into an ovular cage, which …

Continue reading

Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Sewwandi S Rathnayake, Satchal K Erramilli, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2024 Jul 12:2024.07.11.603128. doi: 10.1101/2024.07.11.603128.

PMID: 39026804 PMCID: PMC11257603 DOI: 10.1101/2024.07.11.603128

Pathogenic strains of Clostridium perfringens secrete an enterotoxin (CpE) that causes prevalent, severe, and sometimes deadly gastrointestinal disorders in humans and domesticated animals. CpE …

Continue reading

Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

Commun Biol. 2024 Jun 17;7(1):733. doi: 10.1038/s42003-024-06437-6.

PMID: 38886509 PMCID: PMC11183071 DOI: 10.1038/s42003-024-06437-6

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at …

Continue reading

Development of Luminescent Biosensors for Calprotectin

Tong Lan, Tomasz Slezak, Jinyue Pu, Julia Zinkus-Boltz, Sarbani Adhikari, Joel R. Pekow, Vibha Taneja, Joaquin Zuniga, Itzel A. Gómez-García, Nora Regino-Zamarripa, Mushtaq Ahmed, Shabaana A. Khader, David T. Rubin, Anthony A. Kossiakoff, and Bryan C. Dickinson

ACS Chem. Biol. June 6, 2024 © 2024 American Chemical Society

PMID: 38843544

Continue reading

The Molecular Basis of Human ALKBH3 Mediated RNA N1-methyladenosine (m1A) Demethylation

Lin Zhang, Hong-Chao Duan, Marcin Paduch, Jingyan Hu, Chi Zhang, Yajuan Mu, Houwen Lin, Chuan He, Anthony A. Kossiakoff, Guifang Jia, Liang Zhang

Zitierweise: Angew. Chem. Int. Ed. 2024, 63, e202313900

PMID: 38158383 DOI: 10.1002/ange.202313900

N1-methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, and the distribution and dynamics …

Continue reading

Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP

Hongtao Liu, Dariusz Zakrzewicz, Kamil Nosol, Rossitza N Irobalieva, Somnath Mukherjee, Rose Bang-Sørensen, Nora Goldmann, Sebastian Kunz, Lorenzo Rossi, Anthony A Kossiakoff, Stephan Urban, Dieter Glebe, Joachim Geyer, Kaspar P Locher

Nature Communications volume 15, Article number: 2476 (2024)

PMID: 38509088 PMCID: PMC10954734 DOI: 10.1038/s41467-024-46706-w

Cellular entry of …

Continue reading

Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains

Szymon P. Kordon, Kristina Cechova, Sumit J. Bandekar, Katherine Leon, Przemysław Dutka, Gracie Siffer, Anthony A. Kossiakoff, Reza Vafabakhsh, and Demet Araç

bioRxiv 2024 Feb 26

PMID: 38464178 DOI: 10.1101/2024.02.25.581807

Adhesion G Protein-Coupled Receptors (aGPCRs) are key cell-adhesion molecules involved in numerous physiological functions. aGPCRs have large multi-domain …

Continue reading

Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes

Heather A Bruce, Alexander U Singer, Ekaterina V Filippova, Levi L Blazer, Jarrett J Adams, Leonie Enderle, Moshe Ben-David, Elizabeth H Radley, Daniel Y L Mao, Victor Pau, Stephen Orlicky, Frank Sicheri, Igor Kourinov, Shane Atwell, Anthony A Kossiakoff, Sachdev S Sidhu

Protein Sci. 2023 Nov 9:e4824.

PMID: 37945533 DOI: …

Continue reading

Structural and molecular basis of choline uptake into the brain by FLVCR2

Rosemary J. Cater, Dibyanti Mukherjee, Eva Gil Iturbe, Satchal K. Erramilli, Ting Chen, Katie Koo, Nicolás Santander Grez, Andrew Reckers, Brian Kloss, Tomasz Gawda, Brendon C. Choy, Zhening Zheng, Oliver B. Clarke, Sook Wah Yee, Anthony A. Kossiakoff, Matthias Quick, Thomas Arnold, Filippo Mancia

bioRxiv. 2023 Oct 5:2023.10.05.561059.

PMID: 37873173 PMCID: PMC10592973 …

Continue reading