Category: publications

Cryo-EM Structure of Phospholipase Cε Defines N-terminal Domains and their Roles in Activity.

Kadidia Samassekou, Elisabeth E. Garland-Kuntz, Vaani Ohri, Isaac J. Fisher, Satchal K. Erramilli,Kaushik Muralidharan, Livia M. Bogdan, Abigail M. Gick, Anthony A. Kossiakoff, Angeline M. Lyon

bioRxiv 2024 September 12

PMID: 39314324 PMCID: PMC11418990 DOI: 10.1101/2024.09.11.612521

Phospholipase Cε (PLCε) increases intracellular Ca 2+ and protein kinase C (PKC) …

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Structural insights into translocation and tailored synthesis of hyaluronan

Ireneusz Górniak, Zachery Stephens, Satchal K. Erramilli, Tomasz Gawda, Anthony A. Kossiakoff & Jochen Zimmer

Nature Structural & Molecular Biology (2024)

PMID: 39322765 DOI: 10.1038/s41594-024-01389-1

Hyaluronan (HA) is an essential component of the vertebrate extracellular matrix. It is a heteropolysaccharide of N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcA) reaching …

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Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE

Yaqi Liu, Chelsea M Brown, Nuno Borges, Rodrigo N Nobre, Satchal Erramilli, Meagan Belcher Dufrisne, Brian Kloss, Sabrina Giacometti, Ana M Esteves, Cristina G Timoteo, Piotr Tokarz, Rosemary Cater, Yasu S Morita, Anthony A Kossiakoff, Helena Santos, Phillip J Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 18

PMID: 39345506 …

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Structural insights into terminal arabinosylation biosynthesis of the mycobacterial cell wall arabinan

Yaqi Liu, Chelsea M. Brown, Satchal Erramilli, Yi-Chia Su, Po-Sen Tseng, Yu-Jen Wang, Nam Ha Duong, Piotr Tokarz, Brian Kloss, Cheng-Ruei Han, Hung-Yu Chen, José Rodrigues, Margarida Archer, Todd L. Lowary, Anthony A. Kossiakoff, Phillip J. Stansfeld, Rie Nygaard, Filippo Mancia

bioRxiv 2024 September 17

PMID: 39345558 PMCID: PMC11429727 DOI: …

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Engineered Protein-G variants for plug-and-play applications

Tomasz Slezak, Kelly M. O’Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. Kossiakoff

bioRxiv 2024 August 08.

PMID: 39211271 PMCID: PMC11361062 DOI: 10.1101/2024.08.06.606809

We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful …

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Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage

Jane E. Lodwick, Rong Shen, Satchal Erramilli, Yuan Xie, Karolina Roganowicz, Anthony A. Kossiakoff, Minglei Zhao

bioRxiv 2024 Jun 27:2024.06.27.601040. doi: 10.1101/2024.06.27.601040.

PMID: 38979142 PMCID: PMC11230398 DOI: 10.1101/2024.06.27.601040

Vault is a massive ribonucleoprotein complex found across Eukaryota. The major vault protein (MVP) oligomerizes into an ovular cage, which …

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Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to Human Receptor, Claudin-4

Sewwandi S Rathnayake, Satchal K Erramilli, Anthony A Kossiakoff, Alex J Vecchio

bioRxiv 2024 Jul 12:2024.07.11.603128. doi: 10.1101/2024.07.11.603128.

PMID: 39026804 PMCID: PMC11257603 DOI: 10.1101/2024.07.11.603128

Pathogenic strains of Clostridium perfringens secrete an enterotoxin (CpE) that causes prevalent, severe, and sometimes deadly gastrointestinal disorders in humans and domesticated animals. CpE …

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Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Satchal K Erramilli, Pawel K Dominik, Chinemerem P Ogbu, Anthony A Kossiakoff, Alex J Vecchio

Commun Biol. 2024 Jun 17;7(1):733. doi: 10.1038/s42003-024-06437-6.

PMID: 38886509 PMCID: PMC11183071 DOI: 10.1038/s42003-024-06437-6

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at …

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Development of Luminescent Biosensors for Calprotectin

Tong Lan, Tomasz Slezak, Jinyue Pu, Julia Zinkus-Boltz, Sarbani Adhikari, Joel R. Pekow, Vibha Taneja, Joaquin Zuniga, Itzel A. Gómez-García, Nora Regino-Zamarripa, Mushtaq Ahmed, Shabaana A. Khader, David T. Rubin, Anthony A. Kossiakoff, and Bryan C. Dickinson

ACS Chem. Biol. June 6, 2024 © 2024 American Chemical Society

PMID: 38843544

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The Molecular Basis of Human ALKBH3 Mediated RNA N1-methyladenosine (m1A) Demethylation

Lin Zhang, Hong-Chao Duan, Marcin Paduch, Jingyan Hu, Chi Zhang, Yajuan Mu, Houwen Lin, Chuan He, Anthony A. Kossiakoff, Guifang Jia, Liang Zhang

Zitierweise: Angew. Chem. Int. Ed. 2024, 63, e202313900

PMID: 38158383 DOI: 10.1002/ange.202313900

N1-methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, and the distribution and dynamics …

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